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apramycin teknova  (Teknova)


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    Structured Review

    Teknova apramycin teknova
    Apramycin Teknova, supplied by Teknova, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/apramycin+teknova/LB+Agar+Plates+with+Apramycin/pm35842866-86-181-182
    Average 91 stars, based on 2 article reviews
    apramycin teknova - by Bioz Stars, 2026-10
    91/100 stars

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    Related Articles

    Virus:

    Article Title: Chromosomal integration of complex DNA constructs using CRAGE and CRAGE-Duet systems.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains TransforMax EC100D pir+ Electrocompetent E. coli Lucigen/Epicenter Cat# ECP09500 E. coli BW29427 University of Illinois #aka WM3064 Chemicals, peptides, and recombinant proteins 2-log DNA ladder New England Biolabs Cat# N0550S 96-well black/clear flat bottom polystyrene NBS microplate Corning Cat# 3651 Agarose UltraPure Invitrogen Cat# 16500-500 Amino acids mix Sunrise Science Products Inc. Cat# 1001 (Continued on next page) 4 STAR Protocols 3, 101546, September 16, 2022 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Apramycin sulfate salt Sigma-Aldrich Cat# A2024-1G Betaine Sigma-Aldrich Cat# B2629-100G CaCl2 solution, 1 M Sigma-Aldrich Cat# 21115 Citric acid monohydrate Sigma-Aldrich Cat# 1909 D-glucose Sigma-Aldrich Cat# G8270 Costar 96-well clear round well plate, sterile with lid Fisher Cat# 07-200-760 Diaminopimelic acid Sigma-Aldrich Cat# 33240-5G Filter membranes, nitrocellulose Sigma-Aldrich Cat# N8395-100EA Gel loading dye, purple (63), no SDS New England Biolabs Cat# B7025S Gene Pulser /MicroPulser electroporation cuvettes, 0.1 cm gap Bio-Rad Cat#1652089 Gibson assembly HiFi HC 1-step kit SGI-DNA Cat# GA1100-4X10 Glass balls (5 mM) VWR Cat# 26396-596 LB agar plate Teknova Cat# L1066 LB agar plate with 50 mg/mL apramycin Teknova Cat# L1066 LB agar plate with 50 mg/mL kanamycin Teknova Cat# L1025 LB agar plate with 200 mg/mL kanamycin Teknova Cat# L1077 LB (Miller’s) broth Growcells Cat# MBLE-7030 Kanamycin sulfate salt Sigma-Aldrich Cat# 60615-5G M9 minimal salts (23) Fisher Cat# A1374401 MgSO4 solution, 1 M Sigma-Aldrich Cat# M3409 Phusion High-Fidelity PCR Master Mix (23) New England Biolabs Cat# M0513S SOC medium New England Biolabs Cat# B9020S Sybr Safe Invitrogen Cat# S33102 TAE, 503 Growcells Cat# MRGF-4210 Trace mineral solution ATCC Cat# MD-TMS Vitamin supplement ATCC Cat# MD-VS Yeast extract Sigma-Aldrich Cat# Y1625 Critical commercial assays Gel extraction kit Promega Cat# A9282 Plasmid midi kit Promega Cat# A2495 Quant-it dsDNA HS assay kit Invitrogen Cat# Q32851 DNeasy blood & tissue Kits QIAGEN Cat# 69506 Experimental models: Organisms/strains Photorhabdus luminescens subsp. laumondii TT01 DSMZ Cat# DSM15139 Pseudomonas simiae WCS417r JGI SB119 Serratia odorifera DSMZ Cat# DSM4582 Oligonucleotides ..

    Recombinant:

    Article Title: Chromosomal integration of complex DNA constructs using CRAGE and CRAGE-Duet systems.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains TransforMax EC100D pir+ Electrocompetent E. coli Lucigen/Epicenter Cat# ECP09500 E. coli BW29427 University of Illinois #aka WM3064 Chemicals, peptides, and recombinant proteins 2-log DNA ladder New England Biolabs Cat# N0550S 96-well black/clear flat bottom polystyrene NBS microplate Corning Cat# 3651 Agarose UltraPure Invitrogen Cat# 16500-500 Amino acids mix Sunrise Science Products Inc. Cat# 1001 (Continued on next page) 4 STAR Protocols 3, 101546, September 16, 2022 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Apramycin sulfate salt Sigma-Aldrich Cat# A2024-1G Betaine Sigma-Aldrich Cat# B2629-100G CaCl2 solution, 1 M Sigma-Aldrich Cat# 21115 Citric acid monohydrate Sigma-Aldrich Cat# 1909 D-glucose Sigma-Aldrich Cat# G8270 Costar 96-well clear round well plate, sterile with lid Fisher Cat# 07-200-760 Diaminopimelic acid Sigma-Aldrich Cat# 33240-5G Filter membranes, nitrocellulose Sigma-Aldrich Cat# N8395-100EA Gel loading dye, purple (63), no SDS New England Biolabs Cat# B7025S Gene Pulser /MicroPulser electroporation cuvettes, 0.1 cm gap Bio-Rad Cat#1652089 Gibson assembly HiFi HC 1-step kit SGI-DNA Cat# GA1100-4X10 Glass balls (5 mM) VWR Cat# 26396-596 LB agar plate Teknova Cat# L1066 LB agar plate with 50 mg/mL apramycin Teknova Cat# L1066 LB agar plate with 50 mg/mL kanamycin Teknova Cat# L1025 LB agar plate with 200 mg/mL kanamycin Teknova Cat# L1077 LB (Miller’s) broth Growcells Cat# MBLE-7030 Kanamycin sulfate salt Sigma-Aldrich Cat# 60615-5G M9 minimal salts (23) Fisher Cat# A1374401 MgSO4 solution, 1 M Sigma-Aldrich Cat# M3409 Phusion High-Fidelity PCR Master Mix (23) New England Biolabs Cat# M0513S SOC medium New England Biolabs Cat# B9020S Sybr Safe Invitrogen Cat# S33102 TAE, 503 Growcells Cat# MRGF-4210 Trace mineral solution ATCC Cat# MD-TMS Vitamin supplement ATCC Cat# MD-VS Yeast extract Sigma-Aldrich Cat# Y1625 Critical commercial assays Gel extraction kit Promega Cat# A9282 Plasmid midi kit Promega Cat# A2495 Quant-it dsDNA HS assay kit Invitrogen Cat# Q32851 DNeasy blood & tissue Kits QIAGEN Cat# 69506 Experimental models: Organisms/strains Photorhabdus luminescens subsp. laumondii TT01 DSMZ Cat# DSM15139 Pseudomonas simiae WCS417r JGI SB119 Serratia odorifera DSMZ Cat# DSM4582 Oligonucleotides ..

    Sterility:

    Article Title: Chromosomal integration of complex DNA constructs using CRAGE and CRAGE-Duet systems.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains TransforMax EC100D pir+ Electrocompetent E. coli Lucigen/Epicenter Cat# ECP09500 E. coli BW29427 University of Illinois #aka WM3064 Chemicals, peptides, and recombinant proteins 2-log DNA ladder New England Biolabs Cat# N0550S 96-well black/clear flat bottom polystyrene NBS microplate Corning Cat# 3651 Agarose UltraPure Invitrogen Cat# 16500-500 Amino acids mix Sunrise Science Products Inc. Cat# 1001 (Continued on next page) 4 STAR Protocols 3, 101546, September 16, 2022 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Apramycin sulfate salt Sigma-Aldrich Cat# A2024-1G Betaine Sigma-Aldrich Cat# B2629-100G CaCl2 solution, 1 M Sigma-Aldrich Cat# 21115 Citric acid monohydrate Sigma-Aldrich Cat# 1909 D-glucose Sigma-Aldrich Cat# G8270 Costar 96-well clear round well plate, sterile with lid Fisher Cat# 07-200-760 Diaminopimelic acid Sigma-Aldrich Cat# 33240-5G Filter membranes, nitrocellulose Sigma-Aldrich Cat# N8395-100EA Gel loading dye, purple (63), no SDS New England Biolabs Cat# B7025S Gene Pulser /MicroPulser electroporation cuvettes, 0.1 cm gap Bio-Rad Cat#1652089 Gibson assembly HiFi HC 1-step kit SGI-DNA Cat# GA1100-4X10 Glass balls (5 mM) VWR Cat# 26396-596 LB agar plate Teknova Cat# L1066 LB agar plate with 50 mg/mL apramycin Teknova Cat# L1066 LB agar plate with 50 mg/mL kanamycin Teknova Cat# L1025 LB agar plate with 200 mg/mL kanamycin Teknova Cat# L1077 LB (Miller’s) broth Growcells Cat# MBLE-7030 Kanamycin sulfate salt Sigma-Aldrich Cat# 60615-5G M9 minimal salts (23) Fisher Cat# A1374401 MgSO4 solution, 1 M Sigma-Aldrich Cat# M3409 Phusion High-Fidelity PCR Master Mix (23) New England Biolabs Cat# M0513S SOC medium New England Biolabs Cat# B9020S Sybr Safe Invitrogen Cat# S33102 TAE, 503 Growcells Cat# MRGF-4210 Trace mineral solution ATCC Cat# MD-TMS Vitamin supplement ATCC Cat# MD-VS Yeast extract Sigma-Aldrich Cat# Y1625 Critical commercial assays Gel extraction kit Promega Cat# A9282 Plasmid midi kit Promega Cat# A2495 Quant-it dsDNA HS assay kit Invitrogen Cat# Q32851 DNeasy blood & tissue Kits QIAGEN Cat# 69506 Experimental models: Organisms/strains Photorhabdus luminescens subsp. laumondii TT01 DSMZ Cat# DSM15139 Pseudomonas simiae WCS417r JGI SB119 Serratia odorifera DSMZ Cat# DSM4582 Oligonucleotides ..

    Electroporation:

    Article Title: Chromosomal integration of complex DNA constructs using CRAGE and CRAGE-Duet systems.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains TransforMax EC100D pir+ Electrocompetent E. coli Lucigen/Epicenter Cat# ECP09500 E. coli BW29427 University of Illinois #aka WM3064 Chemicals, peptides, and recombinant proteins 2-log DNA ladder New England Biolabs Cat# N0550S 96-well black/clear flat bottom polystyrene NBS microplate Corning Cat# 3651 Agarose UltraPure Invitrogen Cat# 16500-500 Amino acids mix Sunrise Science Products Inc. Cat# 1001 (Continued on next page) 4 STAR Protocols 3, 101546, September 16, 2022 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Apramycin sulfate salt Sigma-Aldrich Cat# A2024-1G Betaine Sigma-Aldrich Cat# B2629-100G CaCl2 solution, 1 M Sigma-Aldrich Cat# 21115 Citric acid monohydrate Sigma-Aldrich Cat# 1909 D-glucose Sigma-Aldrich Cat# G8270 Costar 96-well clear round well plate, sterile with lid Fisher Cat# 07-200-760 Diaminopimelic acid Sigma-Aldrich Cat# 33240-5G Filter membranes, nitrocellulose Sigma-Aldrich Cat# N8395-100EA Gel loading dye, purple (63), no SDS New England Biolabs Cat# B7025S Gene Pulser /MicroPulser electroporation cuvettes, 0.1 cm gap Bio-Rad Cat#1652089 Gibson assembly HiFi HC 1-step kit SGI-DNA Cat# GA1100-4X10 Glass balls (5 mM) VWR Cat# 26396-596 LB agar plate Teknova Cat# L1066 LB agar plate with 50 mg/mL apramycin Teknova Cat# L1066 LB agar plate with 50 mg/mL kanamycin Teknova Cat# L1025 LB agar plate with 200 mg/mL kanamycin Teknova Cat# L1077 LB (Miller’s) broth Growcells Cat# MBLE-7030 Kanamycin sulfate salt Sigma-Aldrich Cat# 60615-5G M9 minimal salts (23) Fisher Cat# A1374401 MgSO4 solution, 1 M Sigma-Aldrich Cat# M3409 Phusion High-Fidelity PCR Master Mix (23) New England Biolabs Cat# M0513S SOC medium New England Biolabs Cat# B9020S Sybr Safe Invitrogen Cat# S33102 TAE, 503 Growcells Cat# MRGF-4210 Trace mineral solution ATCC Cat# MD-TMS Vitamin supplement ATCC Cat# MD-VS Yeast extract Sigma-Aldrich Cat# Y1625 Critical commercial assays Gel extraction kit Promega Cat# A9282 Plasmid midi kit Promega Cat# A2495 Quant-it dsDNA HS assay kit Invitrogen Cat# Q32851 DNeasy blood & tissue Kits QIAGEN Cat# 69506 Experimental models: Organisms/strains Photorhabdus luminescens subsp. laumondii TT01 DSMZ Cat# DSM15139 Pseudomonas simiae WCS417r JGI SB119 Serratia odorifera DSMZ Cat# DSM4582 Oligonucleotides ..

    Polymerase Chain Reaction:

    Article Title: Chromosomal integration of complex DNA constructs using CRAGE and CRAGE-Duet systems.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains TransforMax EC100D pir+ Electrocompetent E. coli Lucigen/Epicenter Cat# ECP09500 E. coli BW29427 University of Illinois #aka WM3064 Chemicals, peptides, and recombinant proteins 2-log DNA ladder New England Biolabs Cat# N0550S 96-well black/clear flat bottom polystyrene NBS microplate Corning Cat# 3651 Agarose UltraPure Invitrogen Cat# 16500-500 Amino acids mix Sunrise Science Products Inc. Cat# 1001 (Continued on next page) 4 STAR Protocols 3, 101546, September 16, 2022 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Apramycin sulfate salt Sigma-Aldrich Cat# A2024-1G Betaine Sigma-Aldrich Cat# B2629-100G CaCl2 solution, 1 M Sigma-Aldrich Cat# 21115 Citric acid monohydrate Sigma-Aldrich Cat# 1909 D-glucose Sigma-Aldrich Cat# G8270 Costar 96-well clear round well plate, sterile with lid Fisher Cat# 07-200-760 Diaminopimelic acid Sigma-Aldrich Cat# 33240-5G Filter membranes, nitrocellulose Sigma-Aldrich Cat# N8395-100EA Gel loading dye, purple (63), no SDS New England Biolabs Cat# B7025S Gene Pulser /MicroPulser electroporation cuvettes, 0.1 cm gap Bio-Rad Cat#1652089 Gibson assembly HiFi HC 1-step kit SGI-DNA Cat# GA1100-4X10 Glass balls (5 mM) VWR Cat# 26396-596 LB agar plate Teknova Cat# L1066 LB agar plate with 50 mg/mL apramycin Teknova Cat# L1066 LB agar plate with 50 mg/mL kanamycin Teknova Cat# L1025 LB agar plate with 200 mg/mL kanamycin Teknova Cat# L1077 LB (Miller’s) broth Growcells Cat# MBLE-7030 Kanamycin sulfate salt Sigma-Aldrich Cat# 60615-5G M9 minimal salts (23) Fisher Cat# A1374401 MgSO4 solution, 1 M Sigma-Aldrich Cat# M3409 Phusion High-Fidelity PCR Master Mix (23) New England Biolabs Cat# M0513S SOC medium New England Biolabs Cat# B9020S Sybr Safe Invitrogen Cat# S33102 TAE, 503 Growcells Cat# MRGF-4210 Trace mineral solution ATCC Cat# MD-TMS Vitamin supplement ATCC Cat# MD-VS Yeast extract Sigma-Aldrich Cat# Y1625 Critical commercial assays Gel extraction kit Promega Cat# A9282 Plasmid midi kit Promega Cat# A2495 Quant-it dsDNA HS assay kit Invitrogen Cat# Q32851 DNeasy blood & tissue Kits QIAGEN Cat# 69506 Experimental models: Organisms/strains Photorhabdus luminescens subsp. laumondii TT01 DSMZ Cat# DSM15139 Pseudomonas simiae WCS417r JGI SB119 Serratia odorifera DSMZ Cat# DSM4582 Oligonucleotides ..

    Gel Extraction:

    Article Title: Chromosomal integration of complex DNA constructs using CRAGE and CRAGE-Duet systems.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains TransforMax EC100D pir+ Electrocompetent E. coli Lucigen/Epicenter Cat# ECP09500 E. coli BW29427 University of Illinois #aka WM3064 Chemicals, peptides, and recombinant proteins 2-log DNA ladder New England Biolabs Cat# N0550S 96-well black/clear flat bottom polystyrene NBS microplate Corning Cat# 3651 Agarose UltraPure Invitrogen Cat# 16500-500 Amino acids mix Sunrise Science Products Inc. Cat# 1001 (Continued on next page) 4 STAR Protocols 3, 101546, September 16, 2022 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Apramycin sulfate salt Sigma-Aldrich Cat# A2024-1G Betaine Sigma-Aldrich Cat# B2629-100G CaCl2 solution, 1 M Sigma-Aldrich Cat# 21115 Citric acid monohydrate Sigma-Aldrich Cat# 1909 D-glucose Sigma-Aldrich Cat# G8270 Costar 96-well clear round well plate, sterile with lid Fisher Cat# 07-200-760 Diaminopimelic acid Sigma-Aldrich Cat# 33240-5G Filter membranes, nitrocellulose Sigma-Aldrich Cat# N8395-100EA Gel loading dye, purple (63), no SDS New England Biolabs Cat# B7025S Gene Pulser /MicroPulser electroporation cuvettes, 0.1 cm gap Bio-Rad Cat#1652089 Gibson assembly HiFi HC 1-step kit SGI-DNA Cat# GA1100-4X10 Glass balls (5 mM) VWR Cat# 26396-596 LB agar plate Teknova Cat# L1066 LB agar plate with 50 mg/mL apramycin Teknova Cat# L1066 LB agar plate with 50 mg/mL kanamycin Teknova Cat# L1025 LB agar plate with 200 mg/mL kanamycin Teknova Cat# L1077 LB (Miller’s) broth Growcells Cat# MBLE-7030 Kanamycin sulfate salt Sigma-Aldrich Cat# 60615-5G M9 minimal salts (23) Fisher Cat# A1374401 MgSO4 solution, 1 M Sigma-Aldrich Cat# M3409 Phusion High-Fidelity PCR Master Mix (23) New England Biolabs Cat# M0513S SOC medium New England Biolabs Cat# B9020S Sybr Safe Invitrogen Cat# S33102 TAE, 503 Growcells Cat# MRGF-4210 Trace mineral solution ATCC Cat# MD-TMS Vitamin supplement ATCC Cat# MD-VS Yeast extract Sigma-Aldrich Cat# Y1625 Critical commercial assays Gel extraction kit Promega Cat# A9282 Plasmid midi kit Promega Cat# A2495 Quant-it dsDNA HS assay kit Invitrogen Cat# Q32851 DNeasy blood & tissue Kits QIAGEN Cat# 69506 Experimental models: Organisms/strains Photorhabdus luminescens subsp. laumondii TT01 DSMZ Cat# DSM15139 Pseudomonas simiae WCS417r JGI SB119 Serratia odorifera DSMZ Cat# DSM4582 Oligonucleotides ..

    Plasmid Preparation:

    Article Title: Chromosomal integration of complex DNA constructs using CRAGE and CRAGE-Duet systems.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains TransforMax EC100D pir+ Electrocompetent E. coli Lucigen/Epicenter Cat# ECP09500 E. coli BW29427 University of Illinois #aka WM3064 Chemicals, peptides, and recombinant proteins 2-log DNA ladder New England Biolabs Cat# N0550S 96-well black/clear flat bottom polystyrene NBS microplate Corning Cat# 3651 Agarose UltraPure Invitrogen Cat# 16500-500 Amino acids mix Sunrise Science Products Inc. Cat# 1001 (Continued on next page) 4 STAR Protocols 3, 101546, September 16, 2022 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Apramycin sulfate salt Sigma-Aldrich Cat# A2024-1G Betaine Sigma-Aldrich Cat# B2629-100G CaCl2 solution, 1 M Sigma-Aldrich Cat# 21115 Citric acid monohydrate Sigma-Aldrich Cat# 1909 D-glucose Sigma-Aldrich Cat# G8270 Costar 96-well clear round well plate, sterile with lid Fisher Cat# 07-200-760 Diaminopimelic acid Sigma-Aldrich Cat# 33240-5G Filter membranes, nitrocellulose Sigma-Aldrich Cat# N8395-100EA Gel loading dye, purple (63), no SDS New England Biolabs Cat# B7025S Gene Pulser /MicroPulser electroporation cuvettes, 0.1 cm gap Bio-Rad Cat#1652089 Gibson assembly HiFi HC 1-step kit SGI-DNA Cat# GA1100-4X10 Glass balls (5 mM) VWR Cat# 26396-596 LB agar plate Teknova Cat# L1066 LB agar plate with 50 mg/mL apramycin Teknova Cat# L1066 LB agar plate with 50 mg/mL kanamycin Teknova Cat# L1025 LB agar plate with 200 mg/mL kanamycin Teknova Cat# L1077 LB (Miller’s) broth Growcells Cat# MBLE-7030 Kanamycin sulfate salt Sigma-Aldrich Cat# 60615-5G M9 minimal salts (23) Fisher Cat# A1374401 MgSO4 solution, 1 M Sigma-Aldrich Cat# M3409 Phusion High-Fidelity PCR Master Mix (23) New England Biolabs Cat# M0513S SOC medium New England Biolabs Cat# B9020S Sybr Safe Invitrogen Cat# S33102 TAE, 503 Growcells Cat# MRGF-4210 Trace mineral solution ATCC Cat# MD-TMS Vitamin supplement ATCC Cat# MD-VS Yeast extract Sigma-Aldrich Cat# Y1625 Critical commercial assays Gel extraction kit Promega Cat# A9282 Plasmid midi kit Promega Cat# A2495 Quant-it dsDNA HS assay kit Invitrogen Cat# Q32851 DNeasy blood & tissue Kits QIAGEN Cat# 69506 Experimental models: Organisms/strains Photorhabdus luminescens subsp. laumondii TT01 DSMZ Cat# DSM15139 Pseudomonas simiae WCS417r JGI SB119 Serratia odorifera DSMZ Cat# DSM4582 Oligonucleotides ..



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    91
    Teknova apramycin teknova
    Apramycin Teknova, supplied by Teknova, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/apramycin+teknova/LB+Agar+Plates+with+Apramycin/pm35842866-86-181-182
    Average 91 stars, based on 1 article reviews
    apramycin teknova - by Bioz Stars, 2026-10
    91/100 stars
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